Scientific Program

Chair: Wei-Shau Hu (NIH/NCI, USA)

9:00-9:20 Michaela Rumlova: Why is the recruitment of DHX15 into the MPMV particle crucial for genomic RNA packaging?

9:20-9:40 Wei-Shau Hu: The Regulation of HIV-1 Unspliced RNA Functions

9:40-10:00 Redmond Smyth: RNA structures regulating the packaging and translation of HIV-1 spliced viral RNAs

10:00-10:20 Nathan Sherer: Roles for the HIV-1 Gag Nucleocapsid Domain in Viral RNA Subcellular Trafficking and Translation

10:20-10:35 A. Duchon: Live-Cell Imaging Reveals How, When, and Where Gag Selects HIV-1 RNA for Packaging

Chair: Delphine Muriaux (IRIM/CNRS, France)

10:55-11:15 Juan R. Perilla: Computational Insights into pH-Driven Conformational Shifts in HIV-1 Capsid Assembly

11:15 -11:30 Irene Gialdini (Lamb): Cytosolic interactions of HIV-1 GagPol and Gag unveiled by fluorescence fluctuation spectroscopy

11:30-11:45 Don C. Lamb: Gag-GagPol Revisited

11:45-12:00 Ioulia Rouzina: Kinetics of HIV-1 Gag Virion Assembly on the Plasma Membrane

12:00 -12:15 Hélène Dutartre: HTLV-1 hijacks the neuronal SNAP25 protein to ensure viral transmission

12:15-12:30 Mathias Percipalle: Ultrastructural Insights into Purified HTLV-1 Biofilm by Cryo-Electron Tomography

Chair: Michaela Rumlova (University of Chemistry and Technology, Prague)

13:30-13:50 G.Voth: Insights from Multiscale Computer Simulation: HIV-1 Capsid Assembly and Nuclear Pore Entry

13:50-14:05 L.M. Mansky: Interrogating human retrovirus assembly by comparative analysis

14:05-14:20 Y. Mély: Exploring the role of HIV-1 Gag compact form in viral assembly

14:20-14:35 D. Muriaux: Any role of F-actin depolymerization cofactors in HIV-1 assembly in host CD4 T cells.

14:35-14:50 Benjamin Preece: Distinct Architecture and Dynamics of Gag lattice in Immature HIV-1 Virions versus Gag VLPs Visualized by CryoET and minFLUX Microscopy

14:50-15:05 Virgile Rat: Functional and Structural Characterization of SARS-CoV-2 Virus-Like Particles for Assembly and Entry Mechanism Studies

Chair: Saveez Saffarian (University of Utah, USA)

15:30-16:00 Hans-Georg Kräusslich: Perspective Talk: HIV-1 Maturation: What, How and Why

16:00-16:20 Margaret Johnson: Predicting lattice remodeling dynamics from Gag binding kinetics

16:20-16:40 Dominik Hrebík: The conserved HIV-1 spacer peptide 2 triggers matrix lattice maturation

16:40-16:55 Juan S. Rey: Structural maturation of the MA lattice is not required for HIV-1 particle infectivity

16:55-17:10 James B. Munro: Probing HIV-1 Gag conformational dynamics inside the virion: A single-molecule FRET based approach to study viral maturation

Chair: Barbara Müller (University Hospital Heidelberg, Germany)

9:00-9:20 Tomas Cihlar: Lenacapavir: The First-in-Class HIV Capsid Inhibitor with Twice Yearly Dosing for HIV Treatment and Prevention

9:20-9:40 EO Freed: Mechanistic Analysis of INSTI Resistance Conferred by NC Mutations

9:40-9:55 Alex B. Kleinpeter: Lenacapavir resistance diminishes HIV-1 adaptability in culture

Chair: Nathan Sherer (University of Wisconsin, USA)

10:20-10:35 D. Chernikova: A novel immature deltaretroviral assembly exhibits an HTLV-1-like NTD structure, while featuring a unique CA-CTD stabilization

10:35-10:50 J.O. Klarhof: Same bolts, different structure: Retroviruses use IP6 in different ways to build different capsids

10:50-11:05 Marcela Pávová: Interplay between DDX3 and HIV-1 capsid during early infection

11:05-11:20 Francesca Di Nunzio: HIV Capsid Dynamics and Lenacapavir’s Role in Refashioning Viral Replication

Chair: Karin Musier-Forsyth (Ohio State University, USA)

Peijun Zhang: The Journey of the HIV-1 Capsid: From Assembly to Nuclear Entry

Chair: Mark Williams (Northeastern University, USA)

17:30-17:45 M. Morse: DNA condensates induced by HIV-1 nucleocapsid protein resist de-assembly to persist beyond fundamental timescale of protein-DNA interaction

17:45-18:00 Gregory Melikian: Interferon-Induced Transmembrane Proteins Exhibit Distinct HIV-1 Restriction Phenotypes When Expressed in Target Cells and Incorporated into Virions

18:00-18:10 Irene Carlon-Andres: Correlative Fluorescence Lifetime and Electron Microscopy to unveil functional states of HIV-1 maturation

Chair: Tomáš Ruml (University of Chemistry and Technology, Prague)

Paul Bieniasz: The multiple roles of CA in HIV-1 replication

Chair: Redmond Smyth (CNRS, Strasbourg, France)

9:00-9:20 Wesley Sundquist: Highly efficient HIV reverse transcription and integration in a cell-free system

9:20-9:35 Karin Musier-Forsyth: Probing Role of Lysyl-tRNA Synthetase in Suppressing cGAS–STING Signaling to Facilitate HIV-1 Immune Evasion

9:35-9:50 Lydia M. Arnold: Unveiling a new role for nucleocapsid in regulating cGAS immune sensing of HIV-1

9:50-10:05 Yong-Hui Zheng: ADAM17 is an HIV-1 Restriction Factor Antagonized by Nef

10:05-10:20 Tomáš Ruml: Why does the matrix protein of Mason-Pfizer monkey virus interact with calmodulin?

Chair: Wesley Sundquist (University of Utah, USA)

10:50-11:05 Ross Larue: Histone Modifications Guiding Protype Foamy Virus Integration Site Selection

11:05-11:25 Sebla B. Kutluay: Cis- and Trans-acting Factors that Mediate Nuclear Retention of Intron-containing HIV-1 Transcripts

11:25-11:45 Vinay K. Pathak: Lenacapavir Disrupts HIV-1 Core Integrity while Stabilizing the Capsid Lattice

11:45-12:00 Ryan C. Burdick: HIV-1 Uncoating Inside Nuclear Speckles Strongly Enhances Integration into Speckle-associated Chromatin Domains

12:00-12:15 Chiara Tomasini: Deciphering the Biogenesis of HIV-induced CPSF6 Puncta